Following biochemistry-related equipment is available in our lab and in shared facilities.
Equipment located in our laboratory:
- Large and small capacity shakers for cell culture
- Microfluidizer (Microfluidics Corp.) for cell lysis
- Large capacity high speed centrifuge for pelleting cells and protein purification (Beckman)
- FPLC system (Biorad NGC) for protein purification and analysis
- Electrophoresis equipment for DNA and protein separation
- Gas chromatograph (SRI)
- Anaerobic chamber (Coy) and Schlenk line for manipulation of air-sensitive proteins
- 2 UV/vis spectrophotometers: 1 Agilent Cary 60 equipped with an 18-cell changer for protein characterization and 1 Vernier Spectrovis Plus located in the glove bag for measuring absorption spectra anaerobically without having to remove samples from the glove bag
- Electroporator (Biorad) and multiple temperature controlled incubators for molecular cloning
- Liquid nitrogen dewars for sample storage
- 2 workstations (1 PC, 1 Mac) for data analysis software, including crystallography software, and physical and virtual storage for data backup
- 7L fermenter for cell culture (Lambda instruments)
- Pipetting robot (Opentrons)
Following equipment is available in shared research facilities at Chapman:
- -80°C freezers
- Circular dichroism spectrophotometer (Jasco J-1500) with Peltier temperature control and 1.6 mT permanent magnet for magnetic circular dichroism spectroscopy.
- Large capacity autoclaves for sterilizing media
- Thermocyclers for molecular cloning
- Real time PCR instrument for cloning and mRNA quantification
- ICP-OES (Agilent) for analysis of metal clusters
- Proteomics core facility
- State-of-the-art X-ray diffractometer for macromolecular crystallography (Bruker Metaljet)
- Liquid handling robot for automated, high throughput crystallization trials
- Fluorimeter (Horiba) for biophysical characterization of proteins
- Stopped flow instrument for monitoring rapid kinetics (Applied Photophysics connected to Cary 60 spectrophotometer)
- Fumehoods
- Gel imagers
- 400 MHz NMR
- Dynamic light scattering (Waytt)
- Absorption and fluorescence plate reader capable of anisotropy measurements (Tecan)
- Confocal microscopy facility