{"id":240,"date":"2019-01-26T21:32:17","date_gmt":"2019-01-26T21:32:17","guid":{"rendered":"https:\/\/sites.chapman.edu\/owenslab\/?page_id=240"},"modified":"2019-01-26T21:32:17","modified_gmt":"2019-01-26T21:32:17","slug":"equipment","status":"publish","type":"page","link":"https:\/\/sites.chapman.edu\/owenslab\/equipment\/","title":{"rendered":"Instrumentation"},"content":{"rendered":"<p>Following biochemistry-related equipment is available in our lab and in shared facilities.<\/p>\n<p><strong>Equipment located in our laboratory:<\/strong><\/p>\n<ul>\n<li>Large and small capacity shakers for cell culture<\/li>\n<li>Microfluidizer (Microfluidics Corp.) for cell lysis<\/li>\n<li>Large capacity high speed centrifuge for pelleting cells and protein purification (Beckman)<\/li>\n<li>FPLC system (Biorad NGC) for protein purification and analysis<\/li>\n<li>Electrophoresis equipment for DNA and protein separation<\/li>\n<li>Gas chromatograph (SRI)<\/li>\n<li>Anaerobic chamber (Coy) and Schlenk line for manipulation of air-sensitive proteins<\/li>\n<li>2 UV\/vis spectrophotometers: 1 Agilent Cary 60 equipped with an 18-cell changer for protein characterization and 1 Vernier Spectrovis Plus located in the glove bag for measuring absorption spectra anaerobically without having to remove samples from the glove bag<\/li>\n<li>Electroporator (Biorad) and multiple temperature controlled incubators for molecular cloning<\/li>\n<li>Liquid nitrogen dewars for sample storage<\/li>\n<li>2 workstations (1 PC, 1 Mac) for data analysis software, including crystallography software, and physical and virtual storage for data backup<\/li>\n<li>7L fermenter for cell culture (Lambda instruments)<\/li>\n<li>Pipetting robot (Opentrons)<\/li>\n<\/ul>\n<p><strong>Following equipment is available in shared research facilities at Chapman:<\/strong><\/p>\n<ul>\n<li>-80\u00b0C freezers<\/li>\n<li>Circular dichroism spectrophotometer (Jasco J-1500) with Peltier temperature control and 1.6 mT permanent magnet for magnetic circular dichroism spectroscopy.<\/li>\n<li>Large capacity autoclaves for sterilizing media<\/li>\n<li>Thermocyclers for molecular cloning<\/li>\n<li>Real time PCR instrument for cloning and mRNA quantification<\/li>\n<li>ICP-OES (Agilent) for analysis of metal clusters<\/li>\n<li>Proteomics core facility<\/li>\n<li>State-of-the-art X-ray diffractometer for macromolecular crystallography (Bruker Metaljet)<\/li>\n<li>Liquid handling robot for automated, high throughput crystallization trials<\/li>\n<li>Fluorimeter (Horiba) for biophysical characterization of proteins<\/li>\n<li>Stopped flow instrument for monitoring rapid kinetics (Applied Photophysics connected to Cary 60 spectrophotometer)<\/li>\n<li>Fumehoods<\/li>\n<li>Gel imagers<\/li>\n<li>400 MHz NMR<\/li>\n<li>Dynamic light scattering (Waytt)<\/li>\n<li>Absorption and fluorescence plate reader capable of anisotropy measurements (Tecan)<\/li>\n<li>Confocal microscopy facility<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Following biochemistry-related equipment is available in our lab and in shared facilities. Equipment located in our laboratory: Large and small capacity shakers for cell culture&#8230;<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-template-fullwidth.php","meta":{"footnotes":""},"class_list":["post-240","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sites.chapman.edu\/owenslab\/wp-json\/wp\/v2\/pages\/240","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.chapman.edu\/owenslab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.chapman.edu\/owenslab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.chapman.edu\/owenslab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.chapman.edu\/owenslab\/wp-json\/wp\/v2\/comments?post=240"}],"version-history":[{"count":0,"href":"https:\/\/sites.chapman.edu\/owenslab\/wp-json\/wp\/v2\/pages\/240\/revisions"}],"wp:attachment":[{"href":"https:\/\/sites.chapman.edu\/owenslab\/wp-json\/wp\/v2\/media?parent=240"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}