{"id":1028,"date":"2022-09-28T01:39:23","date_gmt":"2022-09-28T01:39:23","guid":{"rendered":"https:\/\/sites.chapman.edu\/cilia\/?page_id=1028"},"modified":"2026-03-24T10:37:40","modified_gmt":"2026-03-24T17:37:40","slug":"francisco-nunez","status":"publish","type":"page","link":"https:\/\/sites.chapman.edu\/cilia\/francisco-nunez\/","title":{"rendered":"Francisco J. Nunez"},"content":{"rendered":"<p><a href=\"https:\/\/sites.chapman.edu\/cilia\/cilia\/files\/2022\/11\/profile-for-website.png\"><br \/>\n<\/a><a href=\"https:\/\/sites.chapman.edu\/cilia\/wp-content\/uploads\/sites\/30\/2022\/11\/Francisco-N-2.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1089\" src=\"https:\/\/sites.chapman.edu\/cilia\/cilia\/files\/2022\/11\/Francisco-N-2-258x300.jpg\" alt=\"\" width=\"180\" height=\"209\" srcset=\"https:\/\/sites.chapman.edu\/cilia\/wp-content\/uploads\/sites\/30\/2022\/11\/Francisco-N-2-258x300.jpg 258w, https:\/\/sites.chapman.edu\/cilia\/wp-content\/uploads\/sites\/30\/2022\/11\/Francisco-N-2-880x1024.jpg 880w, https:\/\/sites.chapman.edu\/cilia\/wp-content\/uploads\/sites\/30\/2022\/11\/Francisco-N-2-768x894.jpg 768w, https:\/\/sites.chapman.edu\/cilia\/wp-content\/uploads\/sites\/30\/2022\/11\/Francisco-N-2-1320x1536.jpg 1320w, https:\/\/sites.chapman.edu\/cilia\/wp-content\/uploads\/sites\/30\/2022\/11\/Francisco-N-2.jpg 1650w\" sizes=\"auto, (max-width: 180px) 100vw, 180px\" \/><\/a><\/p>\n<p style=\"margin: 0in;\"><strong>Contact:<\/strong><\/p>\n<p style=\"margin: 0in;\"><strong>Francisco J. Nunez Ph.D.<\/strong><br \/>\nPostdoctoral Fellow (on rotation only at present)<br \/>\nChapman University School of Pharmacy<br \/>\nHarry and Diane Rinker Health Science Campus<br \/>\n9401 Jeronimo Road, Irvine, CA 92618<br \/>\nOffice: (714) 516-5447<br \/>\nEmail: fnunez@chapman.edu<strong><br \/>\n<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>Education\/Training:<\/strong><\/p>\n<ul>\n<li>Aug, 2022 \u2013 present\u00a0 \u00a0 Postdoctoral Fellow (on rotation only at present)<\/li>\n<li>Aug, 2018 \u2013 2022\u00a0 \u00a0 \u00a0 \u00a0 Ph.D., School of Pharmacy, Chapman University<\/li>\n<li>Aug, 2016 \u2013 2018\u00a0 \u00a0 \u00a0 \u00a0 M.Sc., School of Pharmacy, Chapman University<\/li>\n<li>Sept 2013 &#8211; 2015\u00a0 \u00a0 \u00a0 \u00a0 Research Assistant, Affymetrix<\/li>\n<li>Sept, 2009 \u2013 2013\u00a0 \u00a0 \u00a0 \u00a0B.Sc. in Molecular Biology, UC Davis<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>Research interests:<\/strong><\/p>\n<ul>\n<li>Understanding the role of defective\/dysfunctional primary cilia in heart development and function<\/li>\n<li>Studying the role of discrete calcium signaling events and how differentiation between intraciliary and cytosolic calcium signaling events affects heart development<\/li>\n<li>Identifying and validating ciliary biomarkers in blood samples to develop a genetic screening assay for sickle cells<\/li>\n<li>How GPCR and cAMP signaling molecules pre-arrange into discrete cellular compartments or \u201cmicrodomains\u201d to generate unique responses<\/li>\n<li>Utilizing fluorescent biosensors in live cells to study unique cellular signaling events in real time<\/li>\n<li>Graduate work focused on pulmonary diseases such as asthma, COPD and idiopathic fibrosis<\/li>\n<li>Non-genomic signaling of glucocorticoids<\/li>\n<li>Next generation sequencing (NGS)<\/li>\n<li>Confocal microscopy<\/li>\n<\/ul>\n<p><strong>Industry Experience:<\/strong><\/p>\n<p>Worked at Affymetrix on the UK Biobank Lung Exam Variant Evaluation (UK BiLEVE) project, which consisted of genome-wide genotyping study from samples of 50,000 individuals using microarray technology. The study compared rare and common single nucleotide polymorphisms (SNPs) in smokers and non-smokers with different extremes in FEV1 to gain insights into the genetic architecture of lung health and disease.<\/p>\n<p><strong>Peer Reviewed Publications:<\/strong><\/p>\n<ul>\n<li><strong>Nunez FJ,<\/strong> Amirrad F, Sajid MI, et al. Untargeted metabolomics analysis on kidney tissues from mice reveals potential hypoxia biomarkers. <em>Sci Rep<\/em>. 2023;13(1):17516. Published 2023 Oct 16. doi:10.1038\/s41598-023-44629-y. PMID: 37845304.<\/li>\n<li>Thirugnanam K, Gupta A, <strong>Nunez FJ<\/strong>, et al. Brain microvascular endothelial cells possess a second cilium that arises from the daughter centriole. <em>Front Mol Biosci<\/em>. 2023;10:1250016. Published 2023 Nov 6. doi:10.3389\/fmolb.2023.1250016. PMID: 38028541.<\/li>\n<li>Martina Schmidt, Isabella Cattani-Cavalieri, <strong>Francisco J. Nunez<\/strong> and Rennolds S Ostrom. Phosphodiesterase isoforms and cAMP compartments in the development of new therapies for obstructive pulmonary diseases. <span style=\"text-decoration: underline;\">Current Opinion in Pharmacology,<\/span> 51:34-42 \u00b7 Jul 1, 2020.<\/li>\n<li><strong>Francisco J. Nunez<\/strong>, Timothy B. Johnstone, Maia L. Corpuz, Austin G. Kazarian, Omar Tibla, Reynold A. Panettieri, Jr., Cynthia Koziol-White, Moom R. Roosan and Rennolds S Ostrom. Glucocorticoids rapidly activate cAMP production via G\u03b1s to initiate non-genomic signaling that contributes to one-third of their canonical genomic effects. <span style=\"text-decoration: underline;\">Federation of American Societies for Experimental Biology (FASEB)<\/span>, 34(2):2882-2895 \u00b7 Dec 27, 2019.<\/li>\n<li><strong>Francisco J. Nunez<\/strong>, Nancy A. Schulte, David M. Fogel, Joel Mickalski, Stephen I. Rennard, Raymond B. Penn, Myron L. Toews and Rennolds Ostrom. Agonist-specific desensitization of PGE2-stimulated cAMP signaling due to upregulated phosphodiesterase expression in human lung fibroblasts. <span style=\"text-decoration: underline;\">Naunyn-Schmiedeberg&#8217;s Archives of Pharmacology<\/span>, 393(5):843-865 \u00b7 Dec 28, 2019.<\/li>\n<li>Ojiaku CA, Chung E, Parikh V, Williams JK, Schwab A, Fuentes AL, Corpus ML, Lui V, Paek S, Bexiga NM, Narayan S, <strong>Nunez FJ<\/strong>, Ahn K, Ostrom RS, An SS, Panettieri RA Jr. Transforming Growth Factor-\u00df1 Decreases \u00df2-Agonist\u2013induced Relaxation in Human Airway Smooth Muscle. <span style=\"text-decoration: underline;\">American Journal of Respiratory Cell and Molecular Biology<\/span>, 61(2):209-18 \u00b7 Feb 11, 2019.<\/li>\n<li>Neda Riahifard, Saghar Mozaffari, Taibah Aldakhil, <strong>Francisco J. Nunez<\/strong>, Qamar Alshammari, Saud Alshammari, Jason Yamaki, Keykavous Parang and Rakesh Kumar Tiwari. Design, Synthesis, and Evaluation of Amphiphilic Cyclic and Linear Peptides Composed of Hydrophobic and Positively-Charged Amino Acids as Antibacterial Agents. <span style=\"text-decoration: underline;\">Molecules<\/span> 23(10),2722 \u00b7 Oct 22, 2018.<\/li>\n<\/ul>\n<div style=\"width: 584px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-1028-1\" width=\"584\" height=\"464\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/sites.chapman.edu\/cilia\/wp-content\/uploads\/sites\/30\/2024\/03\/lica-cineloop-7e9a4453cd02665d.mp4?_=1\" \/><a href=\"https:\/\/sites.chapman.edu\/cilia\/wp-content\/uploads\/sites\/30\/2024\/03\/lica-cineloop-7e9a4453cd02665d.mp4\">https:\/\/sites.chapman.edu\/cilia\/wp-content\/uploads\/sites\/30\/2024\/03\/lica-cineloop-7e9a4453cd02665d.mp4<\/a><\/video><\/div>\n<p><strong>Video. <\/strong>Color doppler ultrasound technique to measure blood velocity of internal carotid artery in circle of willis from mouse brain.<\/p>\n<p><strong>Video. <\/strong>Human airway smooth muscle cells (HASM) expressing the cAMP Difference Detection in situ (cADDis) biosensor from Montana Molecular. Stimulation with isoproterenol induces increase in intracellular cAMP levels. A decrease in fluorescence indicates that the\u00a0 the cADDis biosensor detects cAMP molecules. Kinetic studies can then be performed on drug responses. Time lapse is 5 minutes, where drug was added at 1 minute and response was seen within seconds.<\/p>\n<div style=\"width: 584px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-1028-2\" width=\"584\" height=\"584\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/sites.chapman.edu\/cilia\/wp-content\/uploads\/sites\/30\/2022\/11\/cADDis-control-PGE2-1.24.19-snapshots.mp4?_=2\" \/><a href=\"https:\/\/sites.chapman.edu\/cilia\/wp-content\/uploads\/sites\/30\/2022\/11\/cADDis-control-PGE2-1.24.19-snapshots.mp4\">https:\/\/sites.chapman.edu\/cilia\/wp-content\/uploads\/sites\/30\/2022\/11\/cADDis-control-PGE2-1.24.19-snapshots.mp4<\/a><\/video><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Contact: Francisco J. Nunez Ph.D. Postdoctoral Fellow (on rotation only at present) Chapman University School of Pharmacy Harry and Diane Rinker Health Science Campus 9401 Jeronimo Road, Irvine, CA 92618 Office: (714) 516-5447 Email: fnunez@chapman.edu &nbsp; Education\/Training: Aug, 2022 \u2013 &hellip; <a href=\"https:\/\/sites.chapman.edu\/cilia\/francisco-nunez\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":63,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-1028","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sites.chapman.edu\/cilia\/wp-json\/wp\/v2\/pages\/1028","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.chapman.edu\/cilia\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.chapman.edu\/cilia\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.chapman.edu\/cilia\/wp-json\/wp\/v2\/users\/63"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.chapman.edu\/cilia\/wp-json\/wp\/v2\/comments?post=1028"}],"version-history":[{"count":0,"href":"https:\/\/sites.chapman.edu\/cilia\/wp-json\/wp\/v2\/pages\/1028\/revisions"}],"wp:attachment":[{"href":"https:\/\/sites.chapman.edu\/cilia\/wp-json\/wp\/v2\/media?parent=1028"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}