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Followup Notes for Week 13
Hi folks -- Good going on the review session this week. You can find the PDF of all that was written right here. And last semester's g (More)
DNA Sequence Merging Note
Hey folks -- A few of you have let me know that the CAP3 site I posted in the notes has gone down. That's a bummer! Here's an alternative site where you can run CAP3: http://mobyle.pasteur.fr/cgi-bin/portal.py#forms::cap3 With this one: (More)
Followup Notes for Week 12
Hey folks -- Your sequences have arrived! As we discussed, you may find them at: http://c164850.ifinch.com The username is "c164850" and the password is "bio209". Your sequences are in the folder "FALL (More)
Followup Notes for Week 11
Hey folks -- As I write this, your samples are in the queue at the core sequencing facility. What's in those inserts? Did you get Jacaranda GAPC after all? Was there contamination? All will be revealed Tuesday... Here's a recap of the plan for (More)
Followup Notes for Week 10
Hey folks -- Notice The RE digest lab writeup due date has been changed from November 10th to November 17th: you have an extra week. Congrats on polishing off all the lab work for the semester in 208L! We saw a lot of GAPC sized bands this week (More)
Followup Notes for Week 9
Hey folks -- At this point, you\'ve got two information-filled samples sitting in the freezer. First, you\'ve got the plasmids you extracted from their hosts, in all their circular glory. Then, you\'ve got your completed digests, with vector cleav (More)
Followup Notes for Week 8
Hey folks -- I hope Tuesday\'s was a transformative experience for you, or at least for your microbes. You can look forward to finding out whether you have successful little clone-farms this coming week. More)
Followup Notes on Week 7
Hey folks – Good going in last week\'s ligation lab. If all went well, your 1 vector:1 insert-rich ligation products have been chilling in the fridge. Post-lab study guide questions are More)
Followup Notes on Week 6
Hey folks -- We\'ve seen what our gels had to say about our PCR prowess and purified those products. Now we move towards cloning those hopefully-GAPC-1/GAPC-2 sequences into some bacterial buddies, beginning with ligation next week. Your second (More)
Followup Notes for Week 5
Hey folks -- Very cool to see so many bands in our gels! Looking good. Your first-round gel photos are posted here. You don\ (More)
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